|::: : Bone Bank|
BONE BANK ORGANIZATION
Department of Orthopaedics & Traumatology,
Madurai Medical College, Govt. Rajaji Hospital, Madurai – 20.
Dean - Prof.A.Rathinavel.M.S.MCh.Phd.,
Medical Superintendent – Prof. S.Vijayaragavan.MD.,
Deputy Superintendent(TCC) - Prof.V.R.Ganesan M.S.Ortho.,D.Ortho.,
Director - Prof. R. Arivasan.M.S.Ortho.,D.Ortho.,
Deputy Director - Prof P.V.Thirumalai Murugan.M.S.,DNB(Ortho),D.Ortho.,
Faculty - Dr.M.Gopi Manohar.DNB(Ortho),D.Ortho.,
All Senior and Junior Residents will actively participate in Bone Banking
Technical manager cum transplant Coordinator - Ms. Jagapriya Sivaraman
Staff In charge : Mrs.R. Sumathi
Nursing Sister : Mrs.K. Punithavathy
: Mrs. R. Shanmuga Priya
: Mrs. P.Nagalakshmi Priya
: Mrs. K.Mahalakshmi.
Technicians (Lab) : Mr.P.Nanthakumar
Basic Equipments for Bone Bank.
1. Ultra Low Temperature Deep Freezer (-80°C)
2. Band Saw
5. Jet Lavage system
6. Sterile containers
7. Basic Bone Bank Set instruments
8. Basic General Surgery instruments
- Periosteum elevators
- Electric oscillating saw
- Bone holders
BONE PROCUREMENT TEAM
The bone procurement team consists of at least two orthopaedic surgeons, two
residents and three technicians.
Sterile cleaning and labelling of the procured material is done on a separate trolley. Each fragment is washed with saline and then specimens taken from each segment tissue is taken for culture (both anaerobic and aerobic) and histopathological examination and then soaked in 2 liters of normal saline containing antiseptic or antibiotic solution for 10-20 minutes and alcohol.
TRANSPORTATION OF PROCURED TISSUES
Upon completion of procurement, all specimen bottles and culture bottles are immediately transported back to the tissue bank. The specimen bottles are stored in the “Quarantine Freezer” or in the “Quarantine Section” of the freezer immediately, whilst the culture bottles are immediately dispatched to the Department of Laboratory Medicine where the culture and sensitivity for aerobic and anaerobic organisms will be performed.
Each specimen is documented using a separate Donor Form (Deceased Donor Form). All results including the culture and sensitivity tests must be meticulously and accurately recorded in - each form. A copy of the consent form must be properly filed for proper documentation.
Processing of Grafts
• Specimens taken from each segment for culture (both anaerobic and aerobic) and histopathological examination
• Remove all the soft tissue and periosteum with a sharp instrument (knife or periosteum elevator)
• Soak the graft in alcohol solution
• Each fragment is washed with copious saline
• Graft soaked in 2 liters of normal saline containing antiseptic or antibiotic solution for 10-20 minutes.
Packaging and labelling of Grafts The grafts are packaged in three sterile plastic pouches /bags in a triple layer fashion and labelled with Graft Number, Type, date of Retrieval and Other details.
The grafts are irradiated at 25 KGy.
Quality Control /Audit An audit is done every year to assess the utilization of allografts. In addition grafts not suitable for use are discarded.
Preparation of graft for Transplantation:
The graft is removed from the packing and bone piece sent for culture sensitivity.The graft is then thawed in normal saline- antibiotic solution for 10- 20 mins.The graft is thereafter washed with copious amount of betadine and saline and antibiotic.The graft is then prepared into required shape and size required and then used in the patients.
The Bone bank functioning is under process and will start functioning shortly.